GSM6239085: RCC4/VHL Nx/ITE HIF-2a ChIP rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EPAS1
Cell type
Cell type Class
Kidney
Cell type
Renal cell carcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
RCC4+VHL renal cell carcinoma cell line
ambient o2 concentration
21.00%
duration of hypoxia
N/A
treatment
ITE 100nM
duration of treatment
0.5 hours
chip antibody
HIF-2a (rabbit polyclonal, PM9)
cell type
renal cell carcinoma cell line
Sequenced DNA Library
library_name
GSM6239085
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies directed against HIF-1a (rabbit polyclonal, PM14), HIF-2a (rabbit polyclonal, PM9), HIF-1b (rabbit polyclonal, Novus Biologicals) or AHR (Cell Signaling 83200). Automated library preparation was performed using the Apollo prep system (Takara, PrepX Comp ILMN 32i DNA Lib 96).